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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats
doi: 10.3389/fendo.2022.1029750
Figure Lengend Snippet: (A) LV total collagen content determined by hydroxyproline assay. (B–F) Western blot analysis of relative protein expression of (B) myocyte enhancer factor-2 (MEF2), (C) pro B-type natriuretic peptide (proBNP) (D) histone deacetylase 4 (HDAC4), (E) pSer 246 -HDAC4 and (F) pSer 246 -HDAC4/HDAC4 ratio. (G) Representative Western blots of detected proteins with estimated molecular weight (kDa) compared to total protein staining with Ponceau S. Data are presented as mean ± SEM and statistical differences as * p < 0.05, **p < 0.01, *** p < 0.001 (one-way ANOVA paired with Tukey´s multiple comparisons test).
Article Snippet: These membranes were incubated with primary antibodies against CaMKIIδ (ab181052, Abcam, UK), Erk5 (#12950, Cell Signaling, USA), GATA-4 (#36966, Cell Signaling, USA), HDAC4 (#5392, Cell Signaling, USA),
Techniques: Hydroxyproline Assay, Western Blot, Expressing, Histone Deacetylase Assay, Molecular Weight, Staining
Journal: Frontiers in Endocrinology
Article Title: Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats
doi: 10.3389/fendo.2022.1029750
Figure Lengend Snippet: Diagrammatic representation of epigenetic and transcriptional control of hypertrophic signaling in heart. (A) Generally accepted protein pathways mediating cardiac hypertrophy. (B) Pathways targeted by quercetin in ZDF (Zucker Diabetic Fatty) rats. Quercetin attenuated expression of transcription factors MEF2, GATA4 and NFAT3 and downregulated upstream effectors HDAC4, calcineurin and Erk5.
Article Snippet: These membranes were incubated with primary antibodies against CaMKIIδ (ab181052, Abcam, UK), Erk5 (#12950, Cell Signaling, USA), GATA-4 (#36966, Cell Signaling, USA), HDAC4 (#5392, Cell Signaling, USA),
Techniques: Expressing